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n1 methylpseudo utp  (Jena Bioscience)


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    Structured Review

    Jena Bioscience n1 methylpseudo utp
    N1 Methylpseudo Utp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n1+methylpseudo+utp/N1-Methylpseudo-UTP/pm41798954-80-31-33
    Average 94 stars, based on 25 article reviews
    n1 methylpseudo utp - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: mRNA-based SARS-CoV-2 vaccines: intracellular processing and aggregation of the encoded spike protein as a mechanistic contributor to cardiac cellular stress
    Article Snippet: The template encoded the following components: T7 RNA promoter sequence adapted for use with CleanCap AG (#N-7113, TriLink BioTechnologies, USA) [TAATACGACTCACTATAAGG] – α-globin 5’-untranslated region – firefly luciferase open reading frame – β-globin 3’-untranslated region – segmented polyadenosine tract [60A:G:60A] ( ). .. The in vitro transcription reaction (40 mM Tris-HCl, 10 mM DTT, 2 mM spermidine, 0.002% Triton X-100, 16.5 mM magnesium acetate, 4 mM CleanCap AG, 5 mM ATP, CTP, GTP (#NU-1014L), N1-methylpseudo-UTP (#NU-890L, Jena Bioscience GmbH, Germany), 0.0002 U/μl inorganic pyrophosphatase (#EF0221, Thermo Fisher Scientific Inc., USA), 1 U/μl RNasin RNase inhibitor (N2511, Promega Corp., USA), 50 μg/ml DNA template, 8 U/μl T7 RNA polymerase (#EP0113, Thermo Fisher Scientific, USA)) was incubated for 6 hours at 37°C. .. The DNA template was subsequently removed by DNase digestion (15 minutes at 37°C, 100 U/ml, TURBO DNase, #AM2239, Thermo Fisher Scientific Inc., USA).

    Article Title: mRNA-based SARS-CoV-2 vaccines: intracellular processing and aggregation of the encoded spike protein as a mechanistic contributor to cardiac cellular stress.
    Article Snippet: The template encoded the following components: T7 RNA promoter sequence adapted for use with CleanCap AG (#N-7113, TriLink BioTechnologies, USA) [TAATACGACTCACTATAAGG] – a-globin 5’-untranslated region – firefly luciferase open reading frame – b-globin 3’-untranslated region – segmented polyadenosine tract [60A:G:60A] (25). .. The in vitro transcription reaction (40 mM Tris-HCl, 10 mM DTT, 2 mM spermidine, 0.002% Triton X-100, 16.5 mM magnesium acetate, 4 mM CleanCap AG, 5 mM ATP, CTP, GTP (#NU-1014L), N1-methylpseudo-UTP (#NU-890L, Jena Bioscience GmbH, Germany), 0.0002 U/μl inorganic pyrophosphatase (#EF0221, Thermo Fisher Scientific Inc., USA), 1 U/μl RNasin RNase inhibitor (N2511, Promega Corp., USA), 50 μg/ml DNA template, 8 U/μl T7 RNA polymerase (#EP0113, Thermo Fisher Scientific, USA)) was incubated for 6 hours at 37°C. .. The DNA template was subsequently removed by DNase digestion (15 minutes at 37°C, 100 U/ml, TURBO DNase, #AM2239, Thermo Fisher Scientific Inc., USA).

    Incubation:

    Article Title: mRNA-based SARS-CoV-2 vaccines: intracellular processing and aggregation of the encoded spike protein as a mechanistic contributor to cardiac cellular stress
    Article Snippet: The template encoded the following components: T7 RNA promoter sequence adapted for use with CleanCap AG (#N-7113, TriLink BioTechnologies, USA) [TAATACGACTCACTATAAGG] – α-globin 5’-untranslated region – firefly luciferase open reading frame – β-globin 3’-untranslated region – segmented polyadenosine tract [60A:G:60A] ( ). .. The in vitro transcription reaction (40 mM Tris-HCl, 10 mM DTT, 2 mM spermidine, 0.002% Triton X-100, 16.5 mM magnesium acetate, 4 mM CleanCap AG, 5 mM ATP, CTP, GTP (#NU-1014L), N1-methylpseudo-UTP (#NU-890L, Jena Bioscience GmbH, Germany), 0.0002 U/μl inorganic pyrophosphatase (#EF0221, Thermo Fisher Scientific Inc., USA), 1 U/μl RNasin RNase inhibitor (N2511, Promega Corp., USA), 50 μg/ml DNA template, 8 U/μl T7 RNA polymerase (#EP0113, Thermo Fisher Scientific, USA)) was incubated for 6 hours at 37°C. .. The DNA template was subsequently removed by DNase digestion (15 minutes at 37°C, 100 U/ml, TURBO DNase, #AM2239, Thermo Fisher Scientific Inc., USA).

    Article Title: mRNA-based SARS-CoV-2 vaccines: intracellular processing and aggregation of the encoded spike protein as a mechanistic contributor to cardiac cellular stress.
    Article Snippet: The template encoded the following components: T7 RNA promoter sequence adapted for use with CleanCap AG (#N-7113, TriLink BioTechnologies, USA) [TAATACGACTCACTATAAGG] – a-globin 5’-untranslated region – firefly luciferase open reading frame – b-globin 3’-untranslated region – segmented polyadenosine tract [60A:G:60A] (25). .. The in vitro transcription reaction (40 mM Tris-HCl, 10 mM DTT, 2 mM spermidine, 0.002% Triton X-100, 16.5 mM magnesium acetate, 4 mM CleanCap AG, 5 mM ATP, CTP, GTP (#NU-1014L), N1-methylpseudo-UTP (#NU-890L, Jena Bioscience GmbH, Germany), 0.0002 U/μl inorganic pyrophosphatase (#EF0221, Thermo Fisher Scientific Inc., USA), 1 U/μl RNasin RNase inhibitor (N2511, Promega Corp., USA), 50 μg/ml DNA template, 8 U/μl T7 RNA polymerase (#EP0113, Thermo Fisher Scientific, USA)) was incubated for 6 hours at 37°C. .. The DNA template was subsequently removed by DNase digestion (15 minutes at 37°C, 100 U/ml, TURBO DNase, #AM2239, Thermo Fisher Scientific Inc., USA).

    other:

    Article Title: Inhalable mRNA Nanoparticle with Enhanced Nebulization Stability and Pulmonary Microenvironment Infiltration.
    Article Snippet: The delivery of mRNA into the lungs is the key to solving infectious and intractable diseases that frequently occur in the lungs.. Since inhalation using a nebulizer is the most promising method for mRNA delivery into the lungs, there have been many attempts toward adapting lipid nanoparticles for mRNA inhalation.. However, conventional lipid nanoparticles, which have shown great effectiveness for systemic delivery of mRNA and intramuscular vaccination, are not effective for pulmonary delivery due to their structural instability during nebulization and their inability to adapt to the pulmonary microenvironment.

    Article Title: Improvement of Therapeutic Effect via Inducing Non-Apoptotic Cell Death Using mRNA-Protection Nanocage.
    Article Snippet: Plasmid & Cloning: RIPK3 plasmid constructs were subcloned from pcDNA3-FLAG-RIPK3 (#78 815; Addgene).

    Immunopeptidomics:

    Article Title: Improvement of Therapeutic Effect via Inducing Non‐Apoptotic Cell Death Using mRNA‐Protection Nanocage
    Article Snippet: .. Also, N1‐Methylpseudo‐UTP(me1Ψ‐UTP) (NU‐890S, Jena Bioscience) was used in the IVT mRNA synthesis process to reduce its immunogenicity potential. ..

    In Vivo:

    Article Title: Lipid Nanoparticles for Broad‐Spectrum Nucleic Acid Delivery
    Article Snippet: Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development.. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described.. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide.

    Modification:

    Article Title: Lipid Nanoparticles for Broad‐Spectrum Nucleic Acid Delivery
    Article Snippet: Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development.. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described.. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide.



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